Biotechnology+I

**Fall 2017 **  **Location**: Bldg 3 Rm 170
 * (BITC 1411) Introduction to Biotechnology I **
 * SECTION I: COURSE INFORMATION **
 * Instructor **: **Jose Ayala BA** Biotechnology, Forensic Sci. and Chemistry
 * Office ** **Phone**: 210 633 9006
 * Tutoring ** **Hours**: M-Th 6:30-8am//, Thurs 345-4:30pm//
 * E-mail **: jose.ayala@brooksacademy.org

An introduction to the field of biotechnology including applications of biotechnology in molecular biology, biochemistry, research, bioethics, and laboratory safe practices in a regulated environment. The course is supplemented with exciting hands-on laboratory exercises, and real-world research and industry applications which enable the student to master basic skills in working in a biotechnology lab; solution preparation, safe handling of hazardous material, nucleic acid isolation, recombinant DNA cloning, PCR and ELISA. The course concludes with a bioscience career exploration including applied research, biomanufacturing, biomedical devices, and clinical trials.
 * //Course Description: //**


 * //Course E //****//nrollment benefits: //**

<span style="font-family: Calibri,sans-serif; font-size: 12pt;">.
 * <span style="font-family: Calibri,sans-serif; font-size: 12pt;">Receivea full year Science Credit
 * <span style="font-family: Calibri,sans-serif; font-size: 12pt;">Optional dual credit earned by escrow with Austin Community College.
 * <span style="font-family: Calibri,sans-serif; font-size: 12pt;">B.A.C.E. certification option

<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Skills: NONE ; Prerequisites: One year of high school biology and recommended one year of high school chemistry or co-requisite.
 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Prerequisites: //**

<span style="font-family: Calibri,sans-serif; font-size: 11pt;">There is both a laboratory and lecture component to this course, which must be taken together. There will be required readings from the textbooks, as well as occasional additional reading assignments from the literature. Both the lecture and laboratory components will require the use of the Internet and Google classroom which is made available to the student. **//An emphasis is placed on active learning strategies for content mastery//**.
 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Instructional Methodology //**//<span style="font-family: Calibri,sans-serif; font-size: 11pt;">: //

<span style="font-family: Calibri,sans-serif; font-size: 11pt;">This course is designed to provide a fun, practical and hands-on exploration into the field of biotechnology. In support of Brooks Academy’s Mission Statement to support skills for “life-long learning”, this course will challenge students to apply critical thinking skills to their readings, class activities, laboratory exercises, and classroom discussions about current topics in biotechnology. Although an emphasis is placed on each student’s personal responsibility for constructing their new knowledge, opportunities for working collaboratively with groups will also be provided. This course is the first course in a 2 year Biotechnology Program and can also a serve as a 4th year science. <span style="font-family: Calibri,sans-serif; font-size: 11pt;">The Common Course Objectives for this course can be found here: []
 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Course Rationale //**
 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Common Course Objectives //**


 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Academic Student Learning Outcomes: //**
 * 1) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">1. Students will demonstrate verbally and in writing knowledge of the field of biotechnology (including the historical development of the field) and applications of genomics. (Interpersonal skills and Personal Responsibility)
 * 2) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">2. Utilizing the S.I. system of units, students will use a variety of laboratory tools, and equipment to learn about and develop basic lab skills such as pipetting, preparing solutions, and weighing and measuring. (Empirical and Quantitative Skills)
 * 3) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">3. Students will demonstrate verbally, and in writing, knowledge of DNA, RNA, and protein structures.(Critical Thinking Skills)
 * 4) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">4. Students will use technology to isolate, amplify, and detect DNA and proteins.
 * 5) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">5. Students will demonstrate, both verbally and in writing, knowledge of scientific theory related to biotechnology techniques.
 * 6) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">6. Students will be able to demonstrate their understanding of and to utilize proper laboratory procedures within a regulated environment.
 * 7) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">7. Students will consider, discuss and debate current ethical and legal issues in biotechnology.(Communication Skills)

<span style="font-family: Calibri,sans-serif; font-size: 11pt;">The competency outcomes for this course reflect skills necessary in the biotechnology workforce which emphasize communication skills, punctuality, and teamwork in addition to molecular biology skills. The Biotechnology Program is accredited by The Texas Skill Standards System administered by the Texas Workforce Investment Council for integrated Skill Standards into the curricula: [|www.tssb.org/ctc/acc]. <span style="font-family: Calibri,sans-serif; font-size: 11pt;">The Texas Skill Standards System is composed of skill standards and credentials for sub-baccalaureate occupations with strong employment and earnings opportunity. For more information see the Texas Skill Standards here: [|www.tssb.org/community-and-technical-colleges] and our website: []
 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Course Student Learning Outcomes //**


 * <span style="font-family: Calibri,sans-serif;">R&D Skill Standards **
 * <span style="font-family: Calibri,sans-serif; font-size: 9pt;">A3 Operate equipment || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">B2 Perform assays and experiments || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">B3 Troubleshoot experiments and equipment || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">B4 Perform data analysis || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">B5 Communicate results || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">C1 Participate in employer-sponsored safety training || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">C3 Identify unsafe conditions and take corrective action ||
 * <span style="font-family: Calibri,sans-serif; font-size: 9pt;">C4 Suggest continuous improvements || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">C5 Coordinate with work team || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">C7 Handle and dispose of hazardous materials || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">C8 Maintain security || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">D1 Maintain lab notebook || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">D Create documents ||  ||
 * <span style="font-family: Calibri,sans-serif;">Biomanufacturing Skill Standards **
 * <span style="font-family: Calibri,sans-serif; font-size: 9pt;">1.1 Follow Standard Operating Procedures (SOPs) || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">1.3 Document and analyze data || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">2.1 Clean laboratory environment || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">2.2 Attend company safety and security training || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">2.3 Report unsafe conditions || <span style="font-family: Calibri,sans-serif; font-size: 9pt;">2.4 Maintain laboratory security ||  ||


 * //<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Materials //**
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Textbooks: **__<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Required: Ellyn Daugherty, Biotechnology: Science for the New Millennium. 2012. EMCParadigm Publications. ISBN: 978-0-76384-284-0 __
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lab Workbook: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> “//BIOL1414 Introduction to Biotechnology Laboratory Manual & Exercise Workbook//”.
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">* **__<span style="font-family: Calibri,sans-serif; font-size: 11pt;">One copy of the lab manual will be supplied __<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> free provided electronically*
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Supplies: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> spiral or composition notebook, fine tip black/blue Sharpie, Pen- black/blue ink.


 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Email: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">You are **//required//** to communicate with your teacher using your school email account ONLY.
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Office 365 notebook and http://ayalascience2100.wikispaces.com: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">You are **//required//** to utilize these for this course.


 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">SECTION II: GRADING SCHEME AND MISSED EXAM POLICY: **
 * 1) 1. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lecture & Lab Exams. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> (30% ) The course is set up in four modules (one each quarter); Each exam will cover topics and concepts covered by lecture, lab and other assigned reading materials for the quarter. The format of these exams will be diverse, and may include short answer, definitions, problems, discussions, and analysis, graphing and multiple choice questions. The hands-on portion format may require the student to identify or use equipment or interpret results.


 * 1) 2. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Homework (10%) **


 * 1) 3. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Class Project/Labs. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> (20%) The class will participate in a team project per quarter to be determined by the class. This project will have short assignments throughout the quarter. Project format will include a project outline, research ideas & development, and a final short presentation and summary. Details on the assignment will be given in class. Assigned lab will be evaluated based on timely completion, experiment prelab, setup, safety protocols followed and tear-down


 * 1) 4. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Soft Skills /Quiz. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">(15 %). You will be graded on preparing for class, active participation in class, team work, punctuality, and safe behavior in the lab. This will cover areas such as technical Lab skills learned and demonstrated for periodic evaluation.


 * 1) 5. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lab Workbook Exercises/Homework. **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> (10%) Students will turn in lab workbook assignments each week. Format and assignments differs based on the exercise but may include creating lab protocols, internet research, data analysis, creating figures and diagrams from lab experiments and filling in forms. Lab workbook exercises are due one week after all the data has been collected for that lab.

<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Exams /Test 30 <span style="font-family: Calibri,sans-serif; font-size: 11pt;">class lecture work 25 <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Quiz 15 <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Homework /Lab reports 10 <span style="font-family: Calibri,sans-serif; font-size: 11pt;"> 2. __<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lab Skills __ <span style="font-family: Calibri,sans-serif; font-size: 14.6667px;">Class Project/Lab skills 25
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">SUMMARY GRADING SCHEME: **
 * 1) __<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Content Knowledge: __
 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Total Lecture Points **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> **100** ( **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">= Grade %) **


 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Grading Scale: 90‑100% = A; 80‑89% = B; 70‑79% = C; 60‑50% = F **


 * __<span style="font-family: Calibri,sans-serif; font-size: 11pt;">SECTION III - CLASS POLICIES __**
 * 1) ** 1. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Graded Assignments **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">: All assignments, including exams will be graded and returned one week after they are submitted for grading. Grades will be available on parent portal. If you find a discrepancy in your graded assignment and the grade posted to Blackboard, please notify me by email immediately so that it can be addressed.


 * 1) **<span style="color: #00000a; font-family: Calibri,sans-serif; font-size: 11pt;">2. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lecture: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> This classroom will be an active learning experience, sometimes referred to as a ‘flipped classroom’. Short lectures will be given as outlined on the schedule, however, most classes will have videos, animations, practice exercises, and laboratory exercises to learn about new topics in biotechnology. **//__Classroom topics will closely follow the schedule so please complete the pre-class assignment *prior* to class to make the most of your classroom experience!__//**


 * 1) **<span style="color: #00000a; font-family: Calibri,sans-serif; font-size: 11pt;">3. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lab: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> The lab exercises are part of the ‘flipped classroom’ and there is a lab exercise performed most classes as outlined in the schedule and in the ‘Lab Manual & Exercise Workbook’. Lab exercises will be performed in groups; however, **__each student__** should take complete, accurate, and clear notes into their own exercise workbook during the lab as they will be handing in their own workbook. **__All__** members of the group are expected to participate in the exercise and to work together.


 * 1) ** 4. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Expectations: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> The purpose of this class is to explore the exciting world of biotechnology! For some of you this could be your future career and is the first course in the Biotechnology Program, and for others this may be a general interest course or a science elective in a degree in a completely different field! It is important students understand that science courses require active participation and attendance in every class. //__To successfully complete this course, regular class attendance, active participation and an additional 6-8 hrs of study time outside of class is needed to be successful in this course.__//


 * 1) ** 5. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Attendance/Class Participation: ** <span style="color: #000000; font-family: Calibri,sans-serif; font-size: 11pt;">Regular and punctual class attendance is expected of all students. <span style="font-family: Calibri,sans-serif; font-size: 11pt;">As with all math and science courses, topics build from each other and a missed class may result in a gap in knowledge that will prevent the student from understanding concepts being covered. //__Class activities provide a significant component by which grades are assessed, and students must attend classes in order to earn these points__//. Attendance will be taken at each class period, and students who are more than 10 minutes late for class will be marked as absent for that day. **//Students with excessive absences may be withdrawn from the course by the instructor.//**


 * 1) ** 6. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lecture & Lab Exams: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Exams are approximately 45 minutes long and will be given in class during the regularly scheduled class time. If you are late to an exam, no extra time will be given. While taking an exam, students may not leave the room until they have completed their exam and turned it in for grading. **//If you have a medical condition that would require you to leave the room during the exam, you are responsible for arranging with the teacher, to take the written portion of the exam before the rest of the class takes it.//**


 * 1) **<span style="color: #00000a; font-family: Calibri,sans-serif; font-size: 11pt;">7. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Missed Exam Policy: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> Make-up exams will only be offered to students who provide the instructor with a written request along with documentation of an emergency. This is up to the discretion of the instructor.


 * 1) **<span style="color: #00000a; font-family: Calibri,sans-serif; font-size: 11pt;">8. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Late Work Policy: ALL **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Lab workbook exercises are due one week after the exercise is complete. Late work is accepted at the discretion of the instructor. **//I rarely accept late work so students don’t fall behind in current/future work, however, there will one workbook exercise amnesty accepted on the last class where a student may submit __one__ workbook for regrading for any reason.//**


 * 1) ** 9. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Safety Statement: Brooks Academy **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> is committed to providing a safe and healthy environment for study and work. You are expected to learn and comply with environmental, health and safety procedures and agree to follow safety policies. Because some health and safety circumstances are beyond our control, we ask that you become familiar with the Emergency Procedures poster and Campus Safety Plan map in each classroom.

<span style="font-family: Calibri,sans-serif; font-size: 11pt;">You are expected to conduct yourself **professionally** with respect and courtesy to all. Anyone who thoughtlessly or intentionally jeopardizes the health or safety of another individual will be immediately dismissed from the day’s activity, may be withdrawn from the class, and/or barred from attending future activities.


 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Laboratory Safety: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;"> Health and safety are paramount values in science classrooms, laboratories, and field activities. Students are expected to learn, understand and comply with environmental, health and safety (EHS) procedures and protocols, and must agree to abide by the ACC science safety policy. Specific safety information for each activity will be discussed at the beginning of the class activity. For those activities that require specific safety training, __a student who is late and misses the safety training will not be able to participate in the activity__. The comprehensive science safety policy can be found at: [|**www.austincc.edu/sci_safe/**] <span style="font-family: Calibri,sans-serif; font-size: 11pt;">. **//Before students may attend the laboratory classes, they must complete all of the following://**
 * 1) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">1. Review the BASE Biology Lab Safety Policy and fill out the safety guide for your campus
 * 2) <span style="font-family: Calibri,sans-serif; font-size: 11pt;">2. Sign the BASE Biology Safety Contract.


 * <span style="font-family: Calibri,sans-serif; font-size: 11pt;">Student use of Organisms policy is found here: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">[] In the Biotechnology Program you may work with many different types of organisms, some of which may include human and animal cell culture, stem cells, plants, bacteria, yeast, fungi and algae.


 * 1) ** 10. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Electronic Devices: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Although the use of electronic devices such as calculators, notepads, laptops and smart phone applications is highly encouraged, students are not to use personal electronic except only with direct permission by teacher**//.//**


 * 1) **<span style="color: #00000a; font-family: Calibri,sans-serif; font-size: 11pt;">11. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Withdrawal Policy: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">It is the responsibility of each student to ensure that his or her name is removed from the dual credit roll should he or she decides to withdraw from the class


 * 1) ** 12. ** **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">Scholastic Dishonesty: **<span style="font-family: Calibri,sans-serif; font-size: 11pt;">A student attending BASE assumes responsibility for conduct compatible with the mission as an educational institution. Students have the responsibility to submit coursework that is the result of their OWN thought, research, or self-expression. Students must follow all instructions given by faculty when taking examinations, placement assessments, tests, quizzes, and evaluations and lab reports. Actions constituting scholastic dishonesty include, but are not limited to, plagiarism, cheating, fabrication, collusion, and falsifying documents. Penalties for scholastic dishonesty will depend upon the nature of the violation and may range from lowering a grade on one assignment to an “F” in the assignment which if habitual may affect the final course negatively.

** Tentative Course Schedule **- 5 - 45 minute class blocks per week

1st 9 weeks:
 * Intro to Biotechnology
 * Lab: Safety and orientation
 * Lab: Micropipetting
 * Lab: Ancient Biotech (Root beer/cheese)
 * Lab: Solutions
 * Lab: DNA Extraction
 * Lab: Dilutions
 * Lab: Dye electrophoresis
 * Lab: Restriction Digest
 * Module 1 Exam

2rd 9 weeks
 * Forensics
 * Lab: PCR
 * Final Exam project
 * BioManufacturing QA/QC
 * Lab: pGlo transformation
 * Lab: Plasmid Purification
 * Module 2 Exam

3th 9 weeks
 * Lab: pGLO purification
 * Lab: pGlo SDS gel
 * Medical and Ag/Pharma Biotech
 * ELISA
 * Got Protein-CRISPR (Transformation or genetic engineering)
 * DNA barcoding
 * Module 3 Exam
 * Review for BACE Certification exam

4th 9 weeks
 * Course Capstone Project

__**Acknowledgement Sheet for Course syllabus – Biotechnology I**__


 * By printing and signing my name and dating this page section and turning it in to the teacher, I acknowledge that I have read the class syllabus and understand the various sections as presented. I also understand that the syllabus can be modified if necessary by the teacher and the revised version will be posted and announced.**

Student. Print Name _

__Student. Signature__ _

__Date__

__Parent Print Name__

__Parent signature__

__Date__