Biotechnology+II

= Adv. Biotechnology Syllabus / Course Contract = = = Course Title: Adv. Biotechnology Room L70 Instructor: Jose Ayala, BA, Email: jose.ayala@brooksacademy.org Phone: 633-9006 Web site: [|http://ayalascience2100.wikispaces.com] Designated Grade Levels: 11-12th Prerequisite/Recommendation: Biology, Chemistry, Algebra I, and Biotech I with "B" avg.

Required Class Materials/Supplies:


 * 2 composition books, pens(only blue or black ink) ||  ||

Course General overview: Students will review the fundamental principles of biotechnology, career pathways and biotechnology business applications (medical, pharmaceutical, and agricultural) as well as explore the topics in more detail. Topics of study include: basic biology and basic chemistry (reviews), Lab Math, lab safety, antiseptic techniques. Equipment use and calibration, Legal Lab Note Book set up and use, SOP’s writing and use, DNA, RNA, and protein: extraction, isolation, and purification; and bioethics. Laboratory activities may be paper based simulations, teacher demonstrations, computer/on-line lab simulations and wet labs reinforce concepts and principles presented. Self-sufficiency and personal responsibility in work habits is required. All Students are required to complete a year-long independent research project.

ASSESSMENT

classwor)............................……………….…… (30%)
Homework.........................................................(10%)

=
Reading assignments and problem sets/review guides for each chapter/unit studied will often include notes, vocabulary, concept mastery problems and short essays as well as critical thinking problems.. All laboratory activities will have a lab write-up, which could include a formal full report, PowerPoint, poster, and/or analysis questions and a class presentation. Details/rubric for labs will be provided at the appropriate time. ===== _(Init) _(Init)

ATTENDANCE
You will receive an “employee” evaluations on your class participation (counts as classwork,lab and participation). Some experiments, this year, are time consuming, have limited perishable resources, and/or have extensive setup time. If an experiment is not finished to a normal stopping point during regular class time for it to continue on the next regular class meeting, it must be finished to that point that same day after school. Otherwise, the student(s) lab results may be made non-existent due to proper procedures not being done in a timely manner. REDO’s are only permitted if time and adequate supplies /material are available. Not finishing a lab in a timely manner WILL affect a student’s Lab grade.
 * The concepts and skills taught in this class are continually building on previous lessons. The REGULAR labs performed are costly and time consuming to prepare. Biotechnology is a VERY expensive science. Therefore, daily attendance is important for students to fully learn and understand the principles for successful labs. Absences will affect the ability of other students to accomplish the learning objectives, since students will frequently be working with partners or in groups. Absences and Tardies will also affect individual and group participation points. ||

Some longer labs will be periodically scheduled for a Saturday morning (approx. 4 hours long) and student attendance is required/mandatory for lab credit. These labs cannot be made up. If a student gives prior notice to teacher on missing a Saturday lab an alternate assignment with a set deadline will be given. The student can earn only earn a maximum of 70% for the alternate assignment. Saturday labs will be pre announced for student /parent planning for transportation.

ADV. BIOTECH COURSE OUTLINE

Semester1: Concepts and Lab Skills: Standard Laboratory Operating Procedures

Lab Math, safety protocols, SOPs, Bioethics equip. use / calibration Biotech project 1 || DNA and Protein structure/function
 * Careers

Scale up, Manufacturing, Marketing Lab 1 Set up legal notebook, SOP’s

Lab 2 lab safety

Lab 3 scientific methodology

Lab 4 Internet career /company research(proj)

Lab 5 Model organism growth

Lab 6 Aseptic techniques, Equipment use and

Lab 9 Solution and dilutions preparations Lab 23 recombinant plasmid / cloning vectors /

restriction enzyme mapping ||
 * Qtr 2

Biotech project 1 || Lab 10 DNA extraction Lab 11 Media prep

Lab 12 DNA isolation (mini prep)

Lab 13 DNA electrophoresis || Semester 2:


 * Qtr 3

Biotech project 2 || Lab 20 Spectrophotometer / Molecular ID ||
 * Qtr 4

Recombinant DNA tech/transformation / Genetic Engineering

Batch process on popcorn production Biotech project 2 || Lab 24 Cell competency / bacterial transformation / selecting transformants

Lab 25 Cell culture, growth, monitoring

Lab 27 Pipelines / Product Studies || I Adv Biotech syllabus